Components

250 UT4 DNA Polymerase, LIC-qualifiedr> 500 µl10X T4DNA Polymerase Buffer

General description

The LIC cloning procedure uses T4 DNA Polymerase to produce specific non-complementary 12–15 base single stranded overhangs. The proper conversion of the insert ends for ligation-independent cloning requires a specific balance between the exonuclease and polymerase activities of T4 DNA Polymerase. Novagen′s T4 DNA Polymerase has been qualified for ligation-independent cloning using a unique assay to verify its performance.

Novagen′s T4 DNA Polymerase has been qualified for ligation-independent cloning using a unique assay to verify its performance.

Legal Information

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Packaging

250 u in Plastic ampoule

Warning

Toxicity: Standard Handling (A)